PB1158 - Inspiration from the Past: Harnessing Ancient Movable Type Printing for Future Personalised Diagnosis of Stroke and Cerebral Thrombosis

Tuesday, June 25, 2024

13:45 – 14:45 ICT

Room: Exhibition Hall

Co-Author(s)

Lining A. Ju, PhD

University of Sydney, Australia
YZ

Yunduo C. Zhao

PhD Student
The University of Sydney
Darlington, New South Wales, Australia
YZ

Yingqi Kaitlyn Zhang

PhD Student
The University of Sydney
Darlington, New South Wales, Australia
Freda Passam, MD PhD

Clinical Academic Haematologist, Group Leader
Haematology Research Group, Charles Perkins Centre, University of Sydney
Camperdown, Sydney, New South Wales, Australia
TA

Timothy Ang

Interventional Vascular Neurologist
Royal Princes Alfred Hospital
Camperdown, New South Wales, Australia
NA

Nurul Abidin

Postdoctoral fellow
The University of Sydney
Darlington, New South Wales, Australia
AS

Allan Sun

PhD Student
The University of Sydney
Darlington, New South Wales, Australia
AN

Arian Nasser

Research Assistant
The University of Sydney
Darlington, New South Wales, Australia

Presenting Author(s)

YL

Yuta Liu

Background: Cerebral venous sinus thrombosis (CVST) is receiving growing attention due to its association with stroke and COVID-19 vaccine-induced immune thrombotic thrombocytopenia. CVST remains poorly understood due to highly heterogenous Virchow’s triad of altered blood flow, endothelial dysfunction, and hypercoagulability, which vary from patient to patient. Current diagnostic and surveillance strategies fail to capture these thrombotic determinants in a patient-specific manner.

Aims: To address this, we developed a Vein-Chip platform that integrates the CVST vascular anatomy from magnetic resonance venography and the associated haemodynamic flow profile using the “Chinese Movable Type”-like soft stereolithography technique.

Methods: The Vein-Chip recapitulates CVST Virchow’s triad, enabling systematic characterisation of venous thrombogenesis with respect to fibrin formation and platelet aggregation.

Results: In contrast to the arterial settings, platelets universally adhere across the entire CVS Vein-Chip, independent of stenotic geometry and flow disturbance. Fibrin propagates along the flow direction but exclusively deposits on the inner vessel wall. In the event of inflammatory endothelial injury, fibrin deposition mirrors the outer vessel wall but still not in the lumen.

Conclusion(s): Our Vein-Chip promises future applications for personalised thrombotic assessment and monitoring. It is inexpensive, rapid, and allows for large-scale biomanufacturing for clinical use.