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Poster Networking

Vascular Biology, Blood Cells, and Immunothrombosis

PB1355 - Aortic endothelial cells response to pulsatile vs continuous flow at high shear stress

Tuesday, June 25, 2024
13:45 – 14:45 ICT
Room: Exhibition Hall

    Presenting Author(s)

  • CL

    Christina Le Tanno

    PhD Student
    Univ. Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1011- EGID, F-59000 Lille, France
    Lille, Nord-Pas-de-Calais, France

    • Co-Author(s)

  • JA

    Johan Abdoul

    PhD
    Univ. Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1011- EGID, F-59000 Lille, France
    Lille, Nord-Pas-de-Calais, France

  • JP

    Juliette Philippe

    MSc
    Univ. Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1011- EGID, F-59000 Lille, France
    Lille, Nord-Pas-de-Calais, France

  • CH

    Chloé Huyghe

    MD
    Univ. Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1011- EGID, F-59000 Lille, France
    Lille, Nord-Pas-de-Calais, France

  • BV

    Bertrand Vaast

    Technician
    Univ. Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1011- EGID, F-59000 Lille, France
    Lille, Nord-Pas-de-Calais, France

  • AD

    Annabelle Dupont

    professor
    Univ. Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1011-EGID, F5900 Lille
    59000 Lille, Nord-Pas-de-Calais, France

  • EJ

    Emmanuelle Jeanpierre

    PharmD
    Univ. Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1011- EGID, F-59000 Lille, France
    Lille, Nord-Pas-de-Calais, France

  • AU

    Alexandre Ung

    MSc
    Univ. Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1011- EGID, F-59000 Lille, France
    Lille, Nord-Pas-de-Calais, France

  • DC

    Delphine Corseaux

    Professor, PhD
    Univ. Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1011- EGID, F-59000 Lille, France
    Lille, Nord-Pas-de-Calais, France

  • AR

    Antoine Rauch

    MD, Professor of Hematology
    Univ. Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1011- EGID, F-59000 Lille, France
    Lille, Nord-Pas-de-Calais, France

  • FV

    Flavien Vincent

    MD, PhD
    Univ. Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1011- EGID, F-59000 Lille, France
    Lille, Nord-Pas-de-Calais, France

  • BS

    Bart Staels

    Head of Unit
    Univ. Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1011- EGID, F-59000 Lille, France
    lille, Nord-Pas-de-Calais, France

  • MR

    Mickael Rosa

    Assistant professor
    CHRU Lille
    Lille, Nord-Pas-de-Calais, France

  • SS

    Sophie Susen, PhD,MD

    Professor of Haematology
    Centre Hospitalier Universitaire de Lille, Université de Lille, Lille, France
    Lille, Nord-Pas-de-Calais, France

Background: Severe heart failure leads to the implantation of a left ventricular assist device (LVAD) in patients waiting for a heart transplant. These pumps induce a change in the blood flow regime: from a physiological pulsatile flow to a pathological and continuous flow with higher shear stress, provoking bleedings, mostly gastrointestinal, in 35% of patients.

Aims: The aim of this study was to submit aortic endothelial cells to continuous and pulsatile flow to understand how the type of flow impacts their phenotype.

Methods: Aortic endothelial cells were cultured under flow using the IBIDI pump system. Cells were preconditionned with a continuous flow at low shear stress (10 dyn/cm2) before applying continuous or pulsatile flow at high shear stress (35 dyn/cm2) for 24 hours. Culture media were collected to measure the von Willebrand factor (VWF) secretion by ELISA and RNA extraction was performed for RNA-sequencing.

Results: Continuous flow condition led to a significant decrease of VWF release without difference in gene expression. Continuous vs pulsatile flow conditions were analyzed by RNA-sequencing. The top 3 genes increased in continuous vs pulsatile flow condition are SERPINB2, IL1RL1, CXCL8. The 3 most significantly enriched biological pathways for upregulated genes are “Positive regulation of apoptotic process”, “response to lipopolysaccharide”, “response to xenobiotic stimulus”. The top 3 genes decreased in continuous vs pulsatile flow are VIPR1, DEPP1, ABCG1 and the 3 most significantly enriched biological pathways for downregulated gene are “Tube morphogenesis”, “negative regulation of cell population proliferation” and “regulation of angiogenesis”. In a network analysis, results indicated that high shear continuous flow led to a decreased gene expression of actors of the Notch pathway an increased expression of genes involved in oxidative stress.

Conclusion(s): This study shows significant impact of continuous vs pulsatile flow on aortic endothelial cells biology at high shear stress in vitro.