PB0600 - Tissue factor, leukemia inhibitory factor , thrombomodulin, D-Dimer, thrombin generation and procoagulant phospholipid dependent clotting time are predictive for in vitro fertilization outcome

Monday, June 24, 2024

13:45 – 14:45 ICT

Room: Exhibition Hall

Presenting Author(s)

Patrick Van Dreden, PhD

Clinical Research Manager
Diagnostica Stago
Gennevilliers, Ile-de-France, France

Co-Author(s)

el

eleftheria lefkou

biologist
INSERM
thessaloniki, Thessaloniki, Greece
pt

panagiotis tzonis

PhD
Genesis Athens Clinic centre of human reproduction, Athens
Athens, Attiki, Greece
ks

kostas sfakianoudis

biologist
Genesis Athens Clinic centre of human reproduction, Athens
Athens, Attiki, Greece
ar

aurélie rousseau

PhD clinical research manager
Diagnostica STAGO
gennevilliers, Ile-de-France, France
MG

Matthieu Grussé

Laboratory Scientist
Diagnostica Stago
Gennevilliers, Ile-de-France, France
GG

Grigorios Gerotziafas

Pr
Sorbonne University, INSERM UMR_S_938, Saint-Antoine Research Center, CRSA, Team "Cancer Biology and Therapeutics", Group "Cancer - Angiogenesis - Haemostasis", Institut Universitaire de Cancérologie, AP-HP, Saint-Antoine Hospital, Paris, France.
Paris, Ile-de-France, France

Background: The link between blood hypercoagulability, endothelial cell activation and infertility or in vitro fertilization (IVF) failure is a puzzling issue. Soluble Leukemia Inhibitory Factor (LIF), an interleukin 6 class cytokine expressed in the trophectoderm of the developing embryo is an important regulator in the establishment of pregnancy. Endoglin (Eng), a co-receptor for TGF-β1 and TGF-β3 is implicated in vascular complications of pregnancy and particularly in preeclampsia

Aims: The study ROADMAP-IVF, evaluated the synergistic prognostic value of LIF and Eng with the biomarkers of cellular hypercoagulability in predicting the endometrial receptivity of fresh IVF cycles

Methods: The ROADMAP-IVF enrolled 40 women eligible for IVF and control group (CG) of 30 healthy women with uncomplicated pregnancies. Blood was collected on day 2 (D2) from the natural cycle and 6 days after hormone treatment initiation corresponding to D8 of the cycle. PPL, tissue factor (TF), thrombomodulin (TM), von Willebrand factor (vWF) and D-Dimers (DDi), Thrombin generation (TG) , P- and E-Selectin, LIF and Eng were measured. The cut-off values been selected based on ROC analysis.

Results: In 11 women pregnancy was confirmed with ultrasound and in 23 women no pregnancy was echographically documented. At D2 and D8 the DDi and LIF were significantly higher in IVFG as compared to the CG. At D2 most of the patients had PPL-ct, DDi and MRI of TG higher than the upper normal limit (UNL). At D8 only TF was significantly increased as compared to the CG. (Table 1).

Conclusion(s): The ROADMAP-IVF study showed that at least one out of four women eligible for IVF present biological evidence of activation of platelets, endothelial cells, blood coagulation or LIF upregulation. The levels of LIF, DDi, P-Selectine, TM and TF as well as the status of thrombin generation and PPL showed a significant predictive value for the IVF outcome.