PB0156 - Establish reference intervals for von Willebrand factor multimers in Chinese adults

Sunday, June 23, 2024

13:45 – 14:45 ICT

Room: Exhibition Hall

Presenting Author(s)

Junwei Yuan, MA

postgraduate
Department of Laboratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Shanghai, Shanghai, China (People's Republic)

Co-Author(s)

Ziqi Zhang

postgraduate
Department of Laboratory Medicine, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China
Shanghai, Shanghai, China (People's Republic)
Yeling Lu, PhD

Department of Laboratory Medicine, Ruijin Hospital, Shanghai Jiaotong University School of Medicine
Shanghai, Shanghai, China (People's Republic)
XW

Xuefeng Wang

Professor
Department of Laboratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China
Shanghai, Shanghai, China (People's Republic)

Background: The vWF multimeric assay is a second-line test used for the diagnosis and differentiation of von Willebrand Disease (VWD). Conventional methods are qualitative approaches, such as Western blots, which are time consuming and lack standardization. A novel semi-automated von Willebrand factor multimer assay is available for clinical use, which has the potential to achieve standardization. The establishment of reference intervals can provide clinicians with more information in diagnosis and management of VWD.

Aims: To determine the reference intervals for von Willebrand Factor (vWF) multimers including low molecular weight (LMWM), intermediate molecular weight (IMWM) multimers and high molecular weight (HMWM) multimers in Chinese adults.

Methods: Fifty-nine healthy individuals with normal level of vWF antigen and activity levels and who underwent a physical examination were included in the study. A semi-automated von Willebrand factor multimer assay, the Hydragel 5 von Willebrand multimer, was applied for the detection of vWF multimers (vWF:MM). reference intervals is calculated using the 2.5th and 97.5th percentiles and 90% confidence intervals (CI).

Results: The reference intervals for vWF:MM were LMWM 11.92-26.46%, IMWM 22.04-36.75%, HMWM 40.99-62.76%. No age-or sex-related differences were observed.

Conclusion(s): The qualification of vWF:MM by sebia can serve as an auxiliary diagnosis of von Willebrand Disease(VWD) subtypes and need to be further standardized.