PB0191 - Plasmin Generation in Healthy Individuals

Background: The kinetics of plasmin generation (PG) and its inhibition during fibrinolysis have been poorly understood, as the assays to quantify these parameters are limited and poorly accessible.

Aims: To assess the capacity of healthy individuals in plasmin generation and its association with race, gender, age, weight, habits, inflammation, lipid, renal and hepatic profile.

Methods: The study was conducted at the hemostasis laboratory at UNICAMP. A questionnaire was applied to acquire demographic data, medications and habits (smoking and alcoholic beverages). For the assessment of plasmin generation capacity, five parameters were obtained: Lag time (LT-PG), Maximum absorbance (MA-PG), Maximum velocity (VM-PG), Area under the curve (AUC-PG) and Time to maximum velocity (TVM-PG). Total cholesterol, LDL, HDL, triglycerides, blood glucose, creatinine, TGO, TGP and C-reactive protein were assessed. Statistical data were performed in SPSS software.

Results: A total of 100 healthy individuals were included in this study. Although the literature shows significant differences between PG among African and European descendants, in this study this difference was not significant. Obesity classified by bone mass index (BMI) had a median of 28 (17-48) and PG was not influenced by it. Interestingly, the age group over 60 years presented significantly higher LT-GP (p=0.009) compared to individuals between 18-30 years. Men presented significantly lower MA-PG (p=0.003), VM-PG (p=0.011) and AUC-PG(p=0.016) when compared to females. Regarding lipids, abnormal results was found in 40% of individuals, however no association was found with PG. C-reactive protein test was abnormal in more than 50% of individuals and showed significantly correlation with MA-PG, MV-PG and AUC-PG.

Conclusion(s): New developments for PG have been shown promising results. For the first time, the PG profile and its association with demographic data and biochemical laboratory markers were evaluated in healthy individuals. Men have lower plasmin generating capacity compared to women and inflammatory markers appear to contribute to plasmin modulation.