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Poster Networking

Coagulation Factors and Co-Factors

PB1112 - The procoagulant signature of cancer cells drives fibrin network formation in tumor microenvironment and impacts its quality. Implications in cancer cell migration

Tuesday, June 25, 2024
13:45 – 14:45 ICT
Room: Exhibition Hall

    Co-Author(s)

  • CH

    Chi Mai Tran Huong

    PhD student
    Diagnostica STAGO
    Paris, Ile-de-France, France

    • Presenting Author(s)

  • Patrick Van Dreden, PhD

    Clinical Research Manager
    Diagnostica Stago
    Gennevilliers, Ile-de-France, France

    • Co-Author(s)

  • el

    eleftheria lefkou

    biologist
    INSERM
    thessaloniki, Thessaloniki, Greece

  • ar

    aurélie rousseau

    PhD clinical research manager
    Diagnostica STAGO
    gennevilliers, Ile-de-France, France

  • EM

    Elisabeth Mbemba

    engineer
    Sorbonne University, Inserm UMR_S_938, Saint-Antoine Research Center, CRSA Paris France
    Paris, Ile-de-France, France

  • NM

    Noémie Mourot

    PhD
    Sorbonne University, Inserm UMR_S_938, Saint-Antoine Research Center, CRSA, Team "Cancer Biology and Therapeutics", Group "Cancer - Angiogenesis - Haemostasis", Institut Universitaire de Cancérologie, AP-HP, Saint-Antoine Hospital,75012 Paris, France.
    paris, Ile-de-France, France

  • FB

    Faltas Beshoy

    PhD
    Sorbonne University, Inserm UMR_S_938, Saint-Antoine Research Center, CRSA, Team "Cancer Biology and Therapeutics", Group "Cancer - Angiogenesis - Haemostasis", Institut Universitaire de Cancérologie, AP-HP, Saint-Antoine Hospital,75012 Paris, France.
    paris, Ile-de-France, France

  • MS

    Michéle Sabbah

    PhD
    Sorbonne University, Inserm UMR_S_938, Saint-Antoine Research Center, CRSA, Team "Cancer Biology and Therapeutics", Group "Cancer - Angiogenesis - Haemostasis", Institut Universitaire de Cancérologie, AP-HP, Saint-Antoine Hospital,75012 Paris, France.
    paris, Ile-de-France, France

  • GG

    Grigorios Gerotziafas

    Pr
    Sorbonne University, INSERM UMR_S_938, Saint-Antoine Research Center, CRSA, Team "Cancer Biology and Therapeutics", Group "Cancer - Angiogenesis - Haemostasis", Institut Universitaire de Cancérologie, AP-HP, Saint-Antoine Hospital, Paris, France.
    Paris, Ile-de-France, France

Background: Cancer cells induce hypercoagulability in the tumoral microenvironment by expressing Tissue Factor (TF).

Aims: We aim to study the impact of the procoagulant signature of cancer cells on the structure of fibrin clot, and the migration of cancer cells within the fibrin network.

Methods: Pancreatic cancer cells BXPC3 and breast cancer cells MCF7, MDA-MB23 were cultured in the presence of normal Platelet Poor Plasma (PPP) (diluted 10% in conditioning media). Their potential to induce thrombin generation and their fibrinolytic activity were assessed. The structure of fibrin clots was analyzed with Scanning Electron Microscopy (SEM). Cancer cells’ mobility with fibrin clot and their interactions with fibrin were observed.

Results: Cancer cells, in presence of PPP, induced fibrin network formation. Cancer cells expressing high procoagulant potency (BXPC3 cells and MDA-MB23 cells), led to dense fibrin network with fine fibers. MCF7 cells with low procoagulant potential led to thick fibers. Exogenous TF enhanced the density of fibrin network formed by MCF7 cells. Cancer cells through their inherent profibrinolytic potential migrated within the fiber scaffold. The BXPC3 and MCF7 cells moved in clusters whereas the MDA-MB231 cells moved individually within the fibrin network.

Conclusion(s): The procoagulant signature of cancer cells is determinant for the formation of fibrin clot network in the microenvironment and the clot’s structure. Original SEM images show the architecture of “bird’s nest”-like fibrin network being in touch with the cell membranes and surrounding cancer cells. Fibrin network constructed by triggering thrombin generation by cancer cells, provides a scaffold for cell migration.