PB1354 - Effect of tinzaparin, apixaban, enoxaparin and quercetin and on the expression of procoagulant properties of cancer cells and endothelial cells exposed to cancer cell derived microvesicles

Background: Flavonoid Quercetin, has some anti-inflammatory and anti-oxidation activity. And exerts a significant proapoptotic effect on cancer cells as well as a protective effect on endothelial cells.

Aims: Explore the effect of quercetin on the procoagulant potential of pancreatic cancer cells (BXPC3) and breast cancer cells (MCF7) as well as its effect on endothelial cells (HUVEC) exposed to cancer cell derived microvesicles (CaCe-dMV) from BXPC3 and MCF7 cells.

Methods: BXPC3, MCF7 and cells HUVEC cells were pretreated with Quercetin and clinically relevant concentrations of antithrombotic agents (apixaban, enoxaparin, or tinzaparin). The thrombin generation process was studied. Crystal violet was used to assess cell viability.

Results: - Quercetin and tinzaparin significantly reduced the viability of BXPC3 or MCF7 cells. Treatment of cancer cells with enoxaparin or apixaban did not significantly affect the viability of BXPC3 or MCF7 cells.
- Exposure of BXPC3 cells to quercetin and tinzaparin as compared to the control experiment resulted in significant prolongation of the lag-time, while no significant changes were observed with enoxaparin and apixaban. (Table 1)
- The lag-time of thrombin generation induced by HUVEC pre-treated with quercetin and exposed to BXPC3 CaCe-dEVs was significantly longer and the peak was significantly lower as compared to those obtained by HUVEC non-treated with quercetin exposed to BXPC3 CaCe-dEVs. The lag-time of HUVEC pre-treated with apixaban and exposed to BXPC3 CaCe-dEVs was similar to that observed in the experiments with HUVEC non-treated with apixaban and exposed to BXPC3 CaCe-dEVs. Pretreatment with either quercetin or apixaban did not significantly modify the effect of HUVEC exposed to MCF7 CaCe-dEVs. (Table 2)

Conclusion(s): This study showed that quercetin prevents endothelial cell activation and possible TF expression upon exposure to a potent stimulus such as CaCe-dEV from aggressive pancreatic cancer cells (BXPC3).Quercetin and tinzaparin decrease the viability of cancer cells.