Faculty Member Islamic Azad University- Tabriz Branch Tabriz, Azarbayjan-e Sharqi, Iran
Background: Thromboembolism, characterized by formation a thrombus in a blood vessel that breaks loose and is carried by the blood stream to plug another vessel. The clot may plug a vessel in the lungs (pulmonary embolism), brain (stroke), gastrointestinal tract, kidneys, or leg. Thromboembolism is a significant cause of morbidity and mortality, especially in adults.
Aims: The aim of this study was to generate a rabbit model of carotid artery thromboembolic occlusion and the subsequent investigating the feasibility of tPA- loaded PLGA microspheres- mediated shock wave thrombolytic therapy in this model.
Methods: Briefly, New Zealand white rabbits were submitted to common carotid artery thromboembolic occlusion by injecting autologous blood clots. Then treatment group underwent catheter based intracorporeal pulsed electrohydraulic shock wave shock wave (15Kv, 0.5 Hz, 150 impulses) thrombolytic therapy accompanied by tPA (0.6 mg/kg)- loaded PLGA (Poly Lactic- Co- Glycolic Acid) microspheres administration.
Results: Results from histopathology, B-mode and color Doppler ultrasonography, showed a significant reduction in the mean value for thrombus content, blood mean velocity and a significant increase in the mean value for blood volume flow at the stenotic region in the treatment group compared with the other groups (P < 0.05).
Conclusion(s): Enhanced anti- thrombolytic effect of tPA, induced by shock wave- based inertial cavitation effect of collapsed microspheres, can cause to reduce the thrombus content and significantly dilate the luminal cross-sectional area of stenosis and lower treatment time in comparison with conventional tPA thrombolytic therapy.
